Isolation, characterization and sequence analysis of a full-length cDNA clone encoding acetohydroxy acid reductoisomerase from spinach chloroplasts
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چکیده
منابع مشابه
Isolation and characterization of a rice full-length cDNA clone encoding a polyubiquitin.
Ubiquitin is a highly conserved 76-residue protein found in both the nucleus and the cytoplasm of a11 eukaryotic cells (Finley and Varshavsky, 1985). The protein has been implicated in many cellular functions, including the selective targeting of cellular proteins for degradation, chromatin structure, and response to heat shock and other stresses (Monia et al., 1990). Many eukaryotic ubiquitin ...
متن کاملNucleotide sequence of a full length cDNA clone encoding a polyubiquitin gene from Pisum sativum.
(1989) Nucleotide sequence of a full length cDNA clone encoding a polyubiquitin gene from Pisum sativum. This document is made available in accordance with publisher policies and may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the URL above for details on accessing the published ver...
متن کاملIsolation and kinetic properties of acetohydroxy acid isomeroreductase from spinach (Spinacia oleracea) chloroplasts overexpressed in Escherichia coli.
Acetohydroxy acid isomeroreductase catalyses a two-step reaction, an alkyl migration and a NADPH-dependent reduction, in the assembly of the carbon skeletons of branched-chain amino acids. Detailed investigations of acetohydroxy acid isomeroreductase aimed at elucidating the biosynthetic pathway of branched-chain amino acids and at designing new inhibitors of the enzyme having herbicidal potenc...
متن کاملIsolation and characterization of a cDNA clone encoding a small wound-inducible protein from Phaseolus vulgaris.
The metabolic activities of plants are dramatically altered upon mechanical injury or pathogen attack. A large number of proteins accumulate at wound or infection sites, including a heterogeneous class of proteins termed pathogenesis-related proteins (Carr and Klessig, 1990). We report here the isolation of a cDNA clone designated PvPR4 encoding a nove1 protein that resembles other pathogenesis...
متن کاملNucleotide sequence of a full length cDNA clone encoding the deoxyribonuclease I from the rat parotid gland.
Monomeric actin interacts with bovine pancreatic DNasel with high affinity (Kb=5xl0 M~') thus forming a stoichiometric 1:1 complex (1). The parotid DNasel has a lower affinity to actin (5.5 x 10 M~') and exhibits no immunologic crossreactivity with the bovine enzyme (2). A parotid specific Xgtll cDNA library has been constructed and screened with a synthetic oligonucleotide sequence deduced fro...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1991
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2770469